Table of Contents
Recombinant DNA Technology
Why Do Genetic Engineering?
Steps in Genetic Engineering
Prokaryote Advantages
Prokaryote Disadvantages
Plasmids
Isolation of Plasmid DNA
Plasmid map
Manipulation of DNA Sequence
Restriction enzymes
Restriction Endonucleases
Restriction Endonucleases
Generating a Plasmid map
Cloning Vectors
Transformation of Bacteria
CaPO4 Transformation
Newer Methods of Transformation
Last Time
Selection of “Correct” Bacteria
Antibiotic Resistance Genes are a Part of Many (Constructed) Plasmids
Blue-White Screening Promega Corp; Madison, WI
DNA Sequencing
Isolation of Gene of Interest
Isolation of Gene of Interest Use of Antibodies
Isolation of Gene of Interest Genomic Library Screening
Hybridization
Isolation of Gene of Interest Polymerase Chain Reaction (PCR)
Summary Figure: Stryer, Biochemistry
Summary Figure after Stryer, Biochemistry
Summary Figure after Stryer, Biochemistry
Summary Figure after Stryer, Biochemistry
Summary Figure after Stryer, Biochemistry
Summary Figure after Stryer, Biochemistry
Summary Figure after Stryer, Biochemistry
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Author: Thomas Wiese
Email: twiese@fhsu.edu
Home Page: http://www.fhsu.edu/chemistry/twiese/home.htm
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