Proteins

Table of Contents

Proteins

Proteins

Solubility

CompositionSimple vs. Conjugated

Shape: Globular vs. Fibrous

Physical Properties

Function

Structure

1o Structure

Protein Purification Starting Material

Protein Purification Salting-in/salting out

Protein Purification Size exclusion chromatography

Protein Purification Ion-exchange chromatography

Protein Purification Affinity chromatography

Protein Purification Electrophoresis

Protein Amino Acid Composition

Protein Sequencing

Protein Sequencing Continued

Protein Sequencing

Protein Sequencing Example

Protein Sequencing Example

Protein Sequencing Example

Protein Sequencing Example

Protein Sequencing Example

Protein Sequencing Example

Much Data, Much Work

The Process Just Described is a Huge Amount of Work

Forces Involved in 1o Structure

Primary Structure Determines Secondary Structure

a-helix

ß-sheet

collagen triple helix

Forces Involved in 2o Structure

3o Structure

Predicting 3o Structure

3o Structure

Protein Folding

Forces Involved in 3o Structure

Denaturation

4o Structure

Forces Involved in 4o Structure

4o Structure and Sickle-cell Anemia

Email: twiese@fhsu.edu

Home Page: http://www.fhsu.edu/chemistry/twiese/home.htm