Dr. Thomas Wiese

Inhibitors and Effectors
Agent	Action	Concentration to use	stock conc.	Solubility
Cytochalasin B	Inhibits (Glc) transport	100 µM	.	ethanol
Phloridzin	Inhibits (Glc) transport	0.5 mM	.	.
Phloretin	Inhibits (Glc) transport	0.5 mM	.	.
Forskolin	Increase [cAMP]	5-10 µM	10 mM	DMSO
PMA	Increase [cAMP]	10-100 nM	.	.
L-PHA	.	1 µg/mL	.	.
Bryostatin	.	low 10-100 pM high 100 nM-1 µM	.	.
Pertussis Toxin	G proteins	100 ng/mL for 18h	.	.
Okadaic acid	Phosphatase 1 & 2A inhibitor	1-10 µM for 24h	100 µM	DMSO
NH₄Cl	Acidify lysosomes	20 mM for 10 min	.	.
Isoproterenol	.	1 µM	.	.
Sommatostatin	.	100 nM	.	.
Actinomycin D	RNA synthesis inhibitor	1-10 µg/mL	.	.
Cycloheximide	protein synthesis inhibitor	100 µM	.	.
Streptolysin O	pore-forming agent	1 µg/mL for 80min	.	.
Cypermethrin	Phosphatase inhibitor	0.1 µM for 24 h	.	.
A23187	Ca²⁺ Ionophore	.	.	.
Sorbinil	Aldose reductase inhibitor	0.4 mM	.	.
Staurosporine	Ser/Thr kinase inhibitor	100 nM	10 µM	DMSO
Quinidine	Ca²⁺ channel blocker	0.5 mM	.	Methanol
Verapamil	Ca²⁺ channel blocker	0.5 mM	.	Methanol
Riampicin	RNA polymerase inhibitor	.	.	.
GTP[gamma]S	stimulate adenylate cyclase	100 µM	.	.
MG132	preoteasome inhibitor	100 µM	.	DMSO
epoxomycin	preoteasome inhibitor	40 µM	.	DMSO
clasto-lactacystin-beta-lactone,	preoteasome inhibitor	40 µM		DMSO
Cucurbitacin I I	STAT3 inhibitor	0.1~10 nM	5 mg/mL	DMSO
Wortmanin	PI-3-kinase inhibitor	~50 nM
bestatin methyl ester	aminopeptidase inhibitor
PD980059	MEK inhibitor	~100 µM
phenylephrine	a1-adrenergic receptor agonist

Last Modified May 9, 2008



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